Earthworm Field Study
Earthworms are recognized as indicators of soil quality and soil management.
Therefore, the need to conduct environmental risk assessment on them is very vital and essential.
Earthworm niche groupings: The orange lines represent the types and depths of the various
- The study design is according to ISO 11268-3:2014 and ISO 23611-1:2018(E).
- The experimental design is a dose response with three treatment groups; a negative control (water treated control), a positive control (reference
substance) and the test item.
- The test is designed as a randomized complete block with at least four replicates for each treatment (10 m * 10 m per plot), with at least 3 m distance between each plot.
Earthworm experimental field design
Earthworms are sampled from the soil by combining two different methods:
2. Allyl-isothiocyanate (AITC) extraction
The following measurement endpoints are obtained:
The results are carefully evaluated for possible differences between the
control, test and reference item treatments.